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LearnMAB425
antibody from R&D Systems
Targeting: TNFRSF1A
CD120a, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR60
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- MAB425 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat TNF RI/TNFRSF1A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse TNF RI in ELISAs and Western blots. In ELISAs, no cross-reactivity was observed with recombinant human (rh) TNF RI, rhTNF RII, recombinant mouse TNF RII, rhTNF-alpha , recombinant rat TNF-alpha , recombinant porcine TNF-alpha , or rhTNF-beta .
- Reactivity
- Mouse, Rat
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P25118- Isotype
- IgG
- Antibody clone number
- 47803
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references FKBP12 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation.
Pharmacokinetic and Pharmacodynamic Characterisation of an Anti-Mouse TNF Receptor 1 Domain Antibody Formatted for In Vivo Half-Life Extension.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Sequential ELISA to profile multiple cytokines from small volumes.
Selective macrophage suppression during sepsis.
Wang Z, Feng J, Yu J, Chen G
Journal of cell science 2019 May 20;132(10)
Journal of cell science 2019 May 20;132(10)
Pharmacokinetic and Pharmacodynamic Characterisation of an Anti-Mouse TNF Receptor 1 Domain Antibody Formatted for In Vivo Half-Life Extension.
Goodall LJ, Ovecka M, Rycroft D, Friel SL, Sanderson A, Mistry P, Davies ML, Stoop AA
PloS one 2015;10(9):e0137065
PloS one 2015;10(9):e0137065
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Osuchowski MF, Welch K, Siddiqui J, Remick DG
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Osuchowski MF, Welch K, Siddiqui J, Remick DG
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Sequential ELISA to profile multiple cytokines from small volumes.
Osuchowski MF, Siddiqui J, Copeland S, Remick DG
Journal of immunological methods 2005 Jul;302(1-2):172-81
Journal of immunological methods 2005 Jul;302(1-2):172-81
Selective macrophage suppression during sepsis.
Ellaban E, Bolgos G, Remick D
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat TNF RI/TNFRSF1A by Western Blot. Western blot shows lysates of C6 rat glioma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB425) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
