MAB430-100

antibody from R&D Systems

Targeting: TNFRSF1A CD120a, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR60

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB430-100

Provider

R&D Systems

Product name

Mouse TNF RI/TNFRSF1A Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD30, recombinant mouse (rm) CD27, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rmEDAR, rhFas, rmFas, rhEDAR, rhGITR, rmGITR, rhHVEM, rhLT beta R, rmLT beta R, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rhTNF RII or rmTNF RII is observed.

Reactivity

Mouse

Host

Hamster

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

55R170

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Cerebral ischemia-induced angiogenesis is dependent on tumor necrosis factor receptor 1-mediated upregulation of α5β1 and αVβ3 integrins.

Huang H, Huang Q, Wang F, Milner R, Li L
Journal of neuroinflammation 2016 Sep 1;13(1):227

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Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation.

Wertz IE, Newton K, Seshasayee D, Kusam S, Lam C, Zhang J, Popovych N, Helgason E, Schoeffler A, Jeet S, Ramamoorthi N, Kategaya L, Newman RJ, Horikawa K, Dugger D, Sandoval W, Mukund S, Zindal A, Martin F, Quan C, Tom J, Fairbrother WJ, Townsend M, Warming S, DeVoss J, Liu J, Dueber E, Caplazi P, Lee WP, Goodnow CC, Balazs M, Yu K, Kolumam G, Dixit VM
Nature 2015 Dec 17;528(7582):370-5

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TLR-mediated secretion of endoplasmic reticulum aminopeptidase 1 from macrophages.

Goto Y, Ogawa K, Nakamura TJ, Hattori A, Tsujimoto M
Journal of immunology (Baltimore, Md. : 1950) 2014 May 1;192(9):4443-52

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IL-1beta augments TNF-alpha-mediated inflammatory responses from lung epithelial cells.

Saperstein S, Chen L, Oakes D, Pryhuber G, Finkelstein J
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2009 May;29(5):273-84

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TLR-2 is upregulated and mobilized to the hepatocyte plasma membrane in the space of Disse and to the Kupffer cells TLR-4 dependently during acute endotoxemia in mice.

Ojaniemi M, Liljeroos M, Harju K, Sormunen R, Vuolteenaho R, Hallman M
Immunology letters 2006 Feb 15;102(2):158-68

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cytotoxicity Induced by TNF-alpha and Neutralization by Mouse TNF RI/TNFRSF1A Antibody. Recombinant Mouse TNF-alpha (Catalog # 410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-alpha (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB430). The ND₅₀ is typically 5-20 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).