500-P143BT-1MG

antibody from Invitrogen Antibodies

Targeting: TNFRSF1A CD120a, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR60

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P143BT-1MG

Provider

Invitrogen Antibodies

Product name

TNFR1 (soluble) Polyclonal Antibody, Biotin, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type I. Anti-Human sTNF Receptor Type I-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human sTNF Receptor Type I by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTNF Receptor Type I (500-P143) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sTNF Receptor Type I. Western Blot: To detect hsTNF-Receptor I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsTNF-Receptor I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P143BT-1MG will be provided as 2 x 500 µg

Reactivity

Human

Host

Rabbit

Conjugate

Biotin

Vial size

2 x 500 µg

Concentration

0.1-1.0 mg/mL

Storage

-20°C

References

Submitted references

Abnormal disulfide-linked oligomerization results in ER retention and altered signaling by TNFR1 mutants in TNFR1-associated periodic fever syndrome (TRAPS).

Lobito AA, Kimberley FC, Muppidi JR, Komarow H, Jackson AJ, Hull KM, Kastner DL, Screaton GR, Siegel RM
Blood 2006 Aug 15;108(4):1320-7

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hsTNF-Receptor I by Western Blot analysis TNFR1 (soluble) Polyclonal Antibody, Biotin (Product # 500-P143BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsTNF-Receptor I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hsTNF-Receptor I by Western Blot analysis TNFR1 (soluble) Polyclonal Antibody, Biotin (Product # 500-P143BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsTNF-Receptor I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Conjugate

Biotin

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human sTNF Receptor Type I by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of TNFR1 (soluble) Polyclonal Antibody, Biotin (Product # 500-P143BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech TNFR1 (soluble) Polyclonal Antibody (Product # 500-P143-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sTNF Receptor Type I.

Conjugate

Biotin