Explore
Validate
LearnAP12727PU-N
antibody from Acris Antibodies GmbH
Targeting: TNFRSF1A
CD120a, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR60
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP12727PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP12727PU-N, RRID:AB_1771476
- Product name
- anti CD120a / TNFR1 pSer299
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser299 of mouse TNFR.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- (LEFT) The anti-Mouse Phospho-TNFR-Ser299 Pab (Cat.#AP12727PU-N) is used in Western blot to detect Mouse Phospho-TNFR-Ser299 in mouse thymus tissue lysate. (RIGHT) Dot blot analysis of anti-Mouse Phospho-TNFR-Ser299 Pab (Cat.#AP12727PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug per ml.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
