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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-18046 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIP55 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is expected to recognize all reported isoforms (NP_054782.2; NP_001014436.1; NP_001116428.1). It is predicted to react with canine and rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HIP55 by a HIP55 monoclonal antibody (Product # PA5-18046) at a concentration of 1 µg/mL. 3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Jurkat cell lysate using Product # PA5-18046 at a concentration of 1.0 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
