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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-51570 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRICKLE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Shear stimulation of FOXC1 and FOXC2 differentially regulates cytoskeletal activity during lymphatic valve maturation.
Norden PR, Sabine A, Wang Y, Demir CS, Liu T, Petrova TV, Kume T
eLife 2020 Jun 8;9
eLife 2020 Jun 8;9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PRICKLE1 in human cerebral cortex and skeletal muscle tissues using PRICKLE1 Polyclonal Antibody (Product # PA5-51570). Corresponding PRICKLE1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PRICKLE1 in human cerebral cortex using PRICKLE1 Polyclonal Antibody (Product # PA5-51570) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PRICKLE1 in human skeletal muscle using PRICKLE1 Polyclonal Antibody (Product # PA5-51570) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. FOXC1 and FOXC2 mediate lymphatic expression of the PCP signaling component and RHOA signaling regulator Prickle1. ( a ) Reduced expression of Prickle1 , Arhgap21 , and Arhgap23 in Foxc1/c2 -compound mutant LECs isolated from the dorsal skin at E15.5. Graph shows RPKM values from RNA-seq analysis. *** denotes p
