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- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- A03668-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Acetyl Coenzyme A Carboxylase/ACACB detection. Tested with WB, IHC-P, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references ACC2 is under-expressed in lung adenocarcinoma and predicts poor clinical outcomes.
Yu FY, Xu Q, Wei QY, Mo HY, Zhong QH, Zhao XY, Lau ATY, Xu YM
Journal of cancer research and clinical oncology 2022 Nov;148(11):3145-3162
Journal of cancer research and clinical oncology 2022 Nov;148(11):3145-3162
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of ACACB using anti-ACACB antibody (A03668-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysatesLane 2: mouse skeletal muscle tissue lysatesAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACACB antigen affinity purified polyclonal antibody (Catalog # A03668-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACACB at approximately 277KD. The expected band size for ACACB is at 277KD.
- Additional image
