PA5-114376

antibody from Invitrogen Antibodies

Targeting: ACACB ACC2, ACCB, HACC275

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-114376

Provider

Invitrogen Antibodies

Product name

ACACB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: rat skeletal muscle tissue, mouse skeletal muscle tissue. IHC: rat testis tissue. Flow: HL-60 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ACACB in paraffin-embedded rat testis tissue. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in ACACB polyclonal antibody (Product # PA5-114376) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of ACACB in HL-60 cells. Samples were blocked with 10% normal goat serum and incubated in ACACB polyclonal antibody (Product # PA5-114376) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 20°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) and unlabeled sample (Red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of ACACB in HL-60 cells. Samples were blocked with 10% normal goat serum and incubated in ACACB polyclonal antibody (Product # PA5-114376) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 20°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) and unlabeled sample (Red line).