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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunoprecipitation [1]
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- Product number
- GTX101150 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101150, RRID:AB_1241105
- Product name
- NFkB p100 antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 (GTX27900) 7.5% SDS PAGE NFkB p100 antibody GTX101150 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE GTX101150 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NFkB p100 antibody [C2C3], C-term detects NFkB p100 protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and NFkB p100 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and NFkB p100 knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p100 antibody [C2C3], C-term (GTX101150) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NFkB p100/p52 antibody immunoprecipitates NFkB p100/p52 protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A. 30 £gg Jurkat whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p100/p52 protein by 2 £gg of NFkB p100/p52 antibody (GTX101150) 5% SDS-PAGE The immunoprecipitated NFkB p100/p52 protein was detected by NFkB p100/p52 antibody (GTX101150) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
