MA5-17217

antibody from Invitrogen Antibodies

Targeting: NFKB2 LYT-10, NF-kB2, p105, p49/p100, p52

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

MA5-17217

Provider

Invitrogen Antibodies

Product name

Anti-NFkB p52 Monoclonal Antibody (9D2)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control for this product is K562 cells.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

9D2

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFkB p52 using anti-NFkB p52 monoclonal antibody (Product # MA5-17217) on (Lane 1) K562 Cell lysate, (Lane 2) A431 Cell lysate, (Lane 3) NIH3T3 Cell lysate and (Lane 4) C6 Cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), NIH/3T3 (Lane 2), A-431 (Lane 3), K-562 (Lane 4), MCF7 (Lane 5), Jurkat (Lane 6), THP-1 (Lane 7) and HCT 116 (Lane 8). The blot was probed with Anti-NFkB p52 Mouse Monoclonal Antibody (Product # MA5-17217, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 52 kDa band corresponding to NFkB p52 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).