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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [8]
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- Product number
- R31570 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NFKB2 Antibody / Nuclear factor NF-kappa-B
- Antibody type
- Polyclonal
- Description
- This highly specific NFKB2 antibody is suitable for use in Western blot/Immunohistochemistry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NFKB2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of NFKB2 antibody and Lane 1: Jurkat; 2: A549; 3: MCF-7; Expected size: 52 and 100KD, depending on sample tested
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of NFKB2 antibody and recombinant human protein (0.5ng)
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human MCF7, 4) human A549, 5) human U-87 MG, 6) mouse lung and 7) mouse RAW264.7 cell lysate with NFKB2 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: NFKB2 antibody testing of human lung cancer tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: NFKB2 antibody testing of human breast cancer tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC(P) staining of mouse intestine tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC(P) staining of rat intestine tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast cancer tissue with NFKB2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer tissue with NFKB2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse intestine tissue with NFKB2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat intestine tissue with NFKB2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
