PAB21621

antibody from Abnova Corporation

Targeting: NFKB2 LYT-10, NF-kB2, p105, p49/p100, p52

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21621

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21621, RRID:AB_10962107

Product name

NFKB2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NFKB2.

Antigen sequence

ICNYEGPAKIEVDLVTHSDPPRAHAHSLVGKQCSE
LGICAVSVGPKDMTAQFNNLGVLHVTKKNMMGTMI
QKLQRQRLRSRPQGLTEAEQRELEQEAKELKKVMD
LSIVRLRFSAFLRASDGSFSLPLKPVISQP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NFKB2 polyclonal antibody (Cat # PAB21621) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with NFKB2 polyclonal antibody (Cat # PAB21621) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil with NFKB2 polyclonal antibody (Cat # PAB21621) shows distinct cytoplasmic positivity in most cells at 1:10-1:20 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)