Ab117894

antibody from Aladdin Scientific

Targeting: NFKB2 LYT-10, NF-kB2, p105, p49/p100, p52

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab117894

Provider

Aladdin Scientific

Product name

Recombinant NFkB p100/NFKB2 Antibody

Antibody type

Recombinant

Description

Rabbit anti Human NFkB p100/NFKB2 Antibody, Recombinant, could be used for WB, IF, ICC and so on.Application:WB: 1/500-1/1000IF: 1/50-1/200

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 746-877


Vial size

100_l,10_l,1ml,50_l

Concentration

0,3 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant NFkB p100/NFKB2 Antibody (Ab117894) - Western Blot All lanes: Recombinant NFkB p100/NFKB2 Antibody (Ab117894) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 97 kDa Observed band size: 110 kDa Exposure time: 10.0 s

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant NFkB p100/NFKB2 Antibody (Ab117894) - Western Blot All lanes: Recombinant NFkB p100/NFKB2 Antibody (Ab117894) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 97 kDa Observed band size: 110 kDa Exposure time: 33.2 s

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant NFkB p100/NFKB2 Antibody (Ab117894) - IF/ICC Immunocytochemistry analysis of NF-KB p100 (green) in HeLa using Recombinant NFkB p100/NFKB2 Antibody (Ab117894), and cells were counterstained with DAPI (blue).