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Western blot
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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA008422 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA008422, RRID:AB_1854434
- Product name
- Anti-NFKB2
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human NFKB2, Gene description: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100), Alternative Gene Names: LYT-10, NF-kB2, p105, p52, Validated applications: ICC, IHC, WB, Uniprot ID: Q00653, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Defining the structure of the NF-ĸB pathway in human immune cells using quantitative proteomic data.
A roadmap of constitutive NF-κB activity in Hodgkin lymphoma: Dominant roles of p50 and p52 revealed by genome-wide analyses
Kok FO, Wang H, Riedlova P, Goodyear CS, Carmody RJ
Cellular signalling 2021 Dec;88:110154
Cellular signalling 2021 Dec;88:110154
A roadmap of constitutive NF-κB activity in Hodgkin lymphoma: Dominant roles of p50 and p52 revealed by genome-wide analyses
de Oliveira K, Kaergel E, Heinig M, Fontaine J, Patone G, Muro E, Mathas S, Hummel M, Andrade-Navarro M, Hübner N, Scheidereit C
Genome Medicine 2016;8(1)
Genome Medicine 2016;8(1)
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NFKB2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human appendix and skeletal muscle tissues using Anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
