14221-1-AP

antibody from Proteintech Group

Targeting: ACTN2

Provider product page for 14221-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [38]
Comments [0]
Validations
Western blot [1]

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Product number: 14221-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14221-1-AP, RRID:AB_2221547
Product name: ACTN2 antibody
Antibody type: Polyclonal
Description: ACTN2 antibody (Cat. #14221-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

ACTN2 protein structure - 14221-1-AP shown in red.

Submitted references Fibroblast-Derived Microfibrillar-Associated Protein 5 Exerts Cardioprotective Effects After Myocardial Infarction by Activating the NOTCH2 Signaling in Cardiomyocytes.

Guo J, Gu Z, Lin M, Tao H, Jiang Y, Zhang R, Jia D, Zhang N, You S, Sun Y, Jia P
Journal of the American Heart Association 2026 Mar 3;15(5):e045111

Essential role of NONO-HOXA1-Wnt axis in cardiomyocyte differentiation.

Feng Z, Gao Y, Gao H, Sun S, Na W, Huang X, Li S, Tan C, Min S, Lu Y, Zhuang Q, Lin S, Ma X, Liu Y, Shou W, Wang M, Wang J, Gu Z, Sheng W, Wu F, Huang G
Nature communications 2026 Jan 24;17(1)

Voluntary Wheel Running Mitigates Disease in an Orai1 Gain-of-Function Mouse Model of Tubular Aggregate Myopathy.

O'Connor TN, Zhao N, Orciuoli HM, Malik S, Brasile A, Pietrangelo L, He M, Groom L, Leigh J, Mahamed Z, Liang C, Protasi F, Dirksen RT
Cells 2025 Sep 4;14(17)

ANXA4 Alleviates Cardiomyocyte Injury Associated With Ischemia-Reperfusion by Interfering With NFκB p50's Transcriptional Activation of RAGE.

Zhao Z, Zhao Y, Zang X, Ren Y, Song W, Wang L, Chen K, Liang E, Zhou Y, Wang X, Niu K
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Sep 30;39(18):e71033

Missense variants in the myosin binding domains of MYBPC3 and MYBPHL impair sarcomere incorporation.

Araujo KN, Cizauskas HE, Yildiz Y, Fritzmann GE, Bui T, Wittenkeller LM, Peña A, Pak TR, Barefield DY
bioRxiv : the preprint server for biology 2025 Oct 1;

The hanging-heart chip: A portable microfluidic device for high-throughput generation of contractile embryonic stem cell-derived cardiac spheroids.

Lai PT, He CK, Li CH, Matahum J, Tang CY, Hsu CH
Bioengineering & translational medicine 2025 May;10(3):e10726

Parkin inhibits iron overload-induced cardiomyocyte ferroptosis by ubiquitinating ACSL4 and modulating PUFA-phospholipids metabolism.

Xiao D, Chang W, Ao X, Ye L, Wu W, Song L, Yuan X, Feng L, Wang P, Wang Y, Jia Y, Tang X, Wang J
Acta pharmaceutica Sinica. B 2025 Mar;15(3):1589-1607

Exercise-Induced Cardiac Lymphatic Remodeling Mitigates Inflammation in the Aging Heart.

Roh K, Li H, Freeman RN, Zazzeron L, Lee A, Zhou C, Shen S, Xia P, Guerra JRB, Sheffield C, Padera TP, Zhou Y, Kim S, Aguirre A, Houstis N, Roh JD, Ichinose F, Malhotra R, Rosenzweig A, Rhee J
Aging cell 2025 Jun;24(6):e70043

MyoMed205 Counteracts Titin Hyperphosphorylation and the Expression of Contraction-Regulating Proteins in a Rat Model of HFpEF.

Vahle B, Schauer A, Augstein A, Jarabo MP, Friedrich J, Barthel P, Männel A, Mangner N, Labeit S, Bowen TS, Linke A, Adams V
Journal of cachexia, sarcopenia and muscle 2025 Jun;16(3):e13843

Phase 1/2 trial of brogidirsen: Dual-targeting antisense oligonucleotides for exon 44 skipping in Duchenne muscular dystrophy.

Komaki H, Takeshita E, Kunitake K, Ishizuka T, Shimizu-Motohashi Y, Ishiyama A, Sasaki M, Yonee C, Maruyama S, Hida E, Aoki Y
Cell reports. Medicine 2025 Jan 21;6(1):101901

Generation of induced pluripotent stem cell lines from five individuals from two families carrying a pathogenic Dutch MYBPC3 founder variant with variable degrees of hypertrophic cardiomyopathy.

van den Dolder FW, Warnaar VAJ, Onderwater YL, Baas AF, Kuster DWD, van der Velden J
Stem cell research 2025 Aug;86:103697

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She H, Scott GR, Fang Y, Zhao Q, Meng F, Qu Y
Molecular biology and evolution 2025 Apr 30;42(5)

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Wang K, Wang Y, Wan H, Wang J, Hu L, Huang S, Sheng M, Wu J, Han X, Yu Y, Chen P, Chen F
Genes & genomics 2024 Sep;46(9):1013-1022

Divergent Regulatory Roles of Transcriptional Variants of the Chicken LDB3 Gene in Muscle Shaping.

Wei C, Niu Y, Chen B, Wang Y, Cai H, Han R, Tian Y, Liu X, Guo W, Kang X, Li Z
Journal of agricultural and food chemistry 2024 May 29;72(21):12240-12250

Modulation of Titin and Contraction-Regulating Proteins in a Rat Model of Heart Failure with Preserved Ejection Fraction: Limb vs. Diaphragmatic Muscle.

Vahle B, Heilmann L, Schauer A, Augstein A, Jarabo MP, Barthel P, Mangner N, Labeit S, Bowen TS, Linke A, Adams V
International journal of molecular sciences 2024 Jun 16;25(12)

Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts.

Hartley B, Bassiouni W, Roczkowsky A, Fahlman R, Schulz R, Julien O
Journal of proteome research 2024 Feb 2;23(2):844-856

MST1R Gene Variants Predispose Individuals to Tetralogy of Fallot.

Feng Z, Huang X, Gao Y, Gao H, Na W, Tan C, Min S, Lu Y, Zhuang Q, Lin S, Ma X, Chen W, Yan W, Sheng W, Huang G
Phenomics (Cham, Switzerland) 2024 Dec;4(6):548-561

Mapping the unicellular transcriptome of the ascending thoracic aorta to changes in mechanosensing and mechanoadaptation during aging.

Rivera CF, Farra YM, Silvestro M, Medvedovsky S, Matz J, Pratama MY, Vlahos J, Ramkhelawon B, Bellini C
Aging cell 2024 Aug;23(8):e14197

Proteolytic degradation of atrial sarcomere proteins underlies contractile defects in atrial fibrillation.

Cizauskas HE, Burnham HV, Panni A, Peña A, Alvarez-Arce A, Davis MT, Araujo KN, Delligatti CE, Edassery S, Kirk JA, Arora R, Barefield DY
American journal of physiology. Heart and circulatory physiology 2024 Aug 1;327(2):H460-H472

Simultaneous loss of skeletal muscle myosin heavy chain IIx and IIb causes severe skeletal muscle hypoplasia in postnatal mice.

Hitachi K, Kiyofuji Y, Yamaguchi H, Nakatani M, Inui M, Tsuchida K
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Jan;37(1):e22692

Exoskeleton Partial-Coated Stem Cells for Infarcted Myocardium Restoring.

He H, Yuan Y, Wu Y, Lu J, Yang X, Lu K, Liu A, Cao Z, Sun M, Yu M, Wang H
Advanced materials (Deerfield Beach, Fla.) 2023 Dec;35(52):e2307169

Application of adipose mesenchymal stem cell-derived exosomes-loaded β-chitin nanofiber hydrogel for wound healing.

Liu Y, Liu Y, Zhao Y, Wu M, Mao S, Cong P, Zou R, Hou M, Jin H, Bao Y
Folia histochemica et cytobiologica 2022;60(2):167-178

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Liang Q, Wang S, Zhou X, Li Y, Xing S, Sha Y, Yang F, Huang W, Liu N, Li Z, Chen Y, Xu Y, Zhu P, Lan F, Sun N
Developmental cell 2022 Nov 21;57(22):2533-2549.e7

Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.

Hulikova A, Park KC, Loonat AA, Gunadasa-Rohling M, Curtis MK, Chung YJ, Wilson A, Carr CA, Trafford AW, Fournier M, Moshnikova A, Andreev OA, Reshetnyak YK, Riley PR, Smart N, Milne TA, Crump NT, Swietach P
Basic research in cardiology 2022 Mar 31;117(1):17

Effects of macrophages on the proliferation and cardiac differentiation of human induced pluripotent stem cells.

Long C, Guo R, Han R, Li K, Wan Y, Xu J, Gong X, Zhao Y, Yao X, Liu J
Cell communication and signaling : CCS 2022 Jul 18;20(1):108

Microskeletal stiffness promotes aortic aneurysm by sustaining pathological vascular smooth muscle cell mechanosensation via Piezo1.

Qian W, Hadi T, Silvestro M, Ma X, Rivera CF, Bajpai A, Li R, Zhang Z, Qu H, Tellaoui RS, Corsica A, Zias AL, Garg K, Maldonado T, Ramkhelawon B, Chen W
Nature communications 2022 Jan 26;13(1):512

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Wang G, Meng X, Wang P, Wang X, Liu G, Wang DA, Fan C
Biomaterials 2022 Dec;291:121908

Identification of differential microRNAs and messenger RNAs resulting from ASXL transcriptional regulator 3 knockdown during during heart development.

Liu ZQ, Cheng M, Fu F, Li R, Han J, Yang X, Deng Q, Li LS, Lei TY, Li DZ, Liao C
Bioengineered 2022 Apr;13(4):9948-9961

Incomplete Assembly of the Dystrophin-Associated Protein Complex in 2D and 3D-Cultured Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Gilbert G, Kadur Nagaraju C, Duelen R, Amoni M, Bobin P, Eschenhagen T, Roderick HL, Sampaolesi M, Sipido KR
Frontiers in cell and developmental biology 2021;9:737840

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Li B, Xiong W, Liang WM, Chiou JS, Lin YJ, Chang ACY
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Xu Y, Xu F, Lv Y, Wang S, Li J, Zhou C, Jiang J, Xie B, He F
Scientific reports 2021 Mar 18;11(1):6374

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Schuldt M, Johnston JR, He H, Huurman R, Pei J, Harakalova M, Poggesi C, Michels M, Kuster DWD, Pinto JR, van der Velden J
Journal of molecular and cellular cardiology 2021 Jan;150:77-90

Huoxin pill prevents acute myocardial ischaemia injury via inhibition of Wnt/β-catenin signaling.

Wang Q, Ma E, Wo D, Chen J, He J, Peng J, Zhu W, Ren DN
Journal of cellular and molecular medicine 2021 Dec;25(24):11053-11062

LncRNA-Mhrt regulates cardiac hypertrophy by modulating the miR-145a-5p/KLF4/myocardin axis.

Xu Y, Luo Y, Liang C, Zhang T
Journal of molecular and cellular cardiology 2020 Feb;139:47-61

Biomimetic electrical stimulation induces rat bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells via TGF-beta 1 in vitro.

He X, Li L, Tang M, Zeng Y, Li H, Yu X
Progress in biophysics and molecular biology 2019 Nov;148:47-53

Possible mechanism of GATA4 inhibiting myocardin activity during cardiac hypertrophy.

Xu Y, Liang C, Luo Y, Xing W, Zhang T
Journal of cellular biochemistry 2019 Jun;120(6):9047-9055

MiR-466h-5p induces expression of myocardin with complementary promoter sequences.

Luo Y, Liang C, Xu Y, Zhang T
Biochemical and biophysical research communications 2019 Jun 18;514(1):187-193

Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?

Petrova IO, Tyganov SA, Mirzoev TM, Tsaturyan AK, Kozlovskaya IB, Shenkman BS
Doklady. Biochemistry and biophysics 2018 Jul;481(1):205-207

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The ACTN2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ACTN2. This antibody recognizes human, mouse, rat antigen. The ACTN2 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 14221-1-AP.pdf

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