R30092

antibody from NSJ Bioreagents

Targeting: ACTN2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R30092

Provider

NSJ Bioreagents

Product name

alpha-Actinin Antibody

Antibody type

Monoclonal

Antigen

Rabbit skeletal alpha-actinin was used as the immunogen for this alpha-Actinin antibody.

Description

Ascites

Reactivity

Human, Mouse, Rat, Rabbit

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

SA-20

Vial size

100 µg

Concentration

Lyophilized; resuspend with 200 ul for 0.5 mg/ml

Storage

After reconstitution, the alpha-Actinin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat heart, 2) rat skeletal muscle, 3) mouse heart, 4) mouse skeletal muscle and 5) human placenta lysate with alpha-Actinin antibody at 0.5ug/ml. Predicted molecular weight ~105 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) mouse heart, 2) mouse skeletal muscle, 3) rat heart and 4) rat skeletal muscle lysate with alpha-Actinin antibody at 1ug/ml. Predicted molecular weight ~105 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast cancer tissue with alpha-Actinin antibody at 1.5ug/ml. Required HIER: boil the sections in 10mM Citrate buffer, pH6, and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse lung tissue with alpha-Actinin antibody at 1.5 ug/ml. Required HIER: boil the sections in 10mM Citrate buffer, pH6, and allow to cool prior to staining.