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- Antigen structure
- References [1]
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- Immunocytochemistry [2]
- Immunohistochemistry [7]
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- Product number
- PA5-27863 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha Actinin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, GL261, C8D30, NIH-3T3, Rat heart. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (94%), Pig (99%), Chicken (98%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.18 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Alterations in the host transcriptome in vitro following Rift Valley fever virus infection.
Pinkham C, Dahal B, de la Fuente CL, Bracci N, Beitzel B, Lindquist M, Garrison A, Schmaljohn C, Palacios G, Narayanan A, Campbell CE, Kehn-Hall K
Scientific reports 2017 Oct 30;7(1):14385
Scientific reports 2017 Oct 30;7(1):14385
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of alpha Actinin 2 was performed in HeLa cells fixed in ice cold MeOH for 5 min. Green: alpha Actinin 2 Polyclonal Antibody (Product # PA5 27863) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Actinin 2 Polyclonal Antibody detects alpha Actinin 2 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: alpha Actinin 2 stained by alpha Actinin 2 Polyclonal Antibody (Product # PA5-27863) diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of alpha Actinin 2 was performed in paraffin-embedded mouse heart tissue using alpha Actinin 2 Polyclonal Antibody (Product # PA5-27863) at a dilution of 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Actinin 2 Polyclonal Antibody detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. alpha Actinin 2 stained by alpha Actinin 2 Polyclonal Antibody (Product # PA5-27863) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Actinin 2 Polyclonal Antibody detects alpha Actinin 2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. alpha Actinin 2 stained by alpha Actinin 2 Polyclonal Antibody (Product # PA5-27863) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Actinin 2 was performed on normal (top) and chronic pancreatitis (bottom) human tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 8-15 minutes in 10mM sodium citrate buffer (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 3% hydrogen peroxide-methanol for 15 min at room temperature. Tissue slides were washed with deionized water and PBS, and then blocked in 3% BSA-PBS for 30 min at room temperature. Tissues were probed with a ACTN2 polyclonal antibody (Product # PA5-27863) diluted 1:500 in 3% BSA-PBS overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Images were taken at 20X magnification. Data courtesy of Antibody Data Exchange Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of alpha Actinin 2 was performed in paraffin-embedded mouse muscle tissue using alpha Actinin 2 Polyclonal Antibody (Product # PA5-27863) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of alpha Actinin 2 was performed in paraffin-embedded rat muscle tissue using alpha Actinin 2 Polyclonal Antibody (Product # PA5-27863) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of alpha Actinin 2 was performed in paraffin-embedded rat heart tissue using alpha Actinin 2 Polyclonal Antibody (Product # PA5-27863) at a dilution of 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
