PA5-29246

antibody from Invitrogen Antibodies

Targeting: ACTN2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29246

Provider

Invitrogen Antibodies

Product name

alpha Actinin 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, NIH-3T3, Rat heart. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (89%), Pig (99%), Chicken (97%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Importance of the type I insulin-like growth factor receptor in HER2, FGFR2 and MET-unamplified gastric cancer with and without Ras pathway activation.

Saisana M, Griffin SM, May FE
Oncotarget 2016 Aug 23;7(34):54445-54462

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of alpha Actinin 2 in Rat tissue extracts (50 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with alpha Actinin 2 Polyclonal antibody (Product # PA5-29246) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using alpha Actinin 2 Polyclonal Antibody (Product # PA5-29246). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299 . 7.5% SDS PAGE. Alpha Actinin 2 Polyclonal Antibody (Product # PA5-29246) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using alpha Actinin 2 Polyclonal Antibody (Product # PA5-29246). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3 . 7.5% SDS PAGE. alpha Actinin 2 Polyclonal Antibody (Product # PA5-29246) diluted at 1:3,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

alpha Actinin 2 antibody detects alpha Actinin 2 protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody alpha Actinin 2 Polyclonal Antibody (Product # PA5-29246) diluted at 1:5,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of alpha Actinin 2 in paraformaldehyde-fixed HeLa cells using an Alpha Actinin 2 polyclonal antibody (Product # PA5-29246) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of alpha actinin 2 was performed on human pancreas tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 8-15 minutes in 10mM sodium citrate buffer (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 3% hydrogen peroxide-methanol for 15 min at room temperature. Tissue slides were washed with deionized water and PBS, and then blocked in 3% BSA-PBS for 30 min at room temperature. Tissues were probed with an ACTN2 polyclonal antibody (Product # PA5-29246) diluted 1:500 in 3% BSA-PBS overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Images were taken at 20X magnification. Data courtesy of Antibody Data Exchange Program.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

alpha Actinin 2 Polyclonal Antibody detects alpha Actinin 2 protein at cytoplasm and membrane on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Alpha Actinin 2 Polyclonal Antibody (Product # PA5-29246) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.