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- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Immunohistochemistry [1]
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- Product number
- MA1104 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-alpha-Actinin Antibody (monoclonal)
- Antibody type
- Monoclonal
- Antigen
- Rabbit skeletal alpha-actinin.
- Description
- Ascites
- Reactivity
- Human, Mouse, Rat, Rabbit
- Vial size
- 100μg/vial
- Concentration
- Lyophilized
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100μg/ml.
Submitted references Leveraging chromatin state transitions for the identification of regulatory networks orchestrating heart regeneration.
Intracellular TMEM16A is necessary for myogenesis of skeletal muscle.
Switching in the expression pattern of actin isoforms marks the onset of contractility and distinct mechanodynamic behavior during cardiomyocyte differentiation.
Nuclear miR-665 aggravates heart failure via suppressing phosphatase and tensin homolog transcription.
Mechanical Ring Interfaces between Adherens Junction and Contractile Actomyosin to Coordinate Entotic Cell-in-Cell Formation.
Optimization and enrichment of induced cardiomyocytes derived from mouse fibroblasts by reprogramming with cardiac transcription factors.
Cordero J, Elsherbiny A, Wang Y, Jürgensen L, Constanty F, Günther S, Boerries M, Heineke J, Beisaw A, Leuschner F, Hassel D, Dobreva G
Nucleic acids research 2024 May 8;52(8):4215-4233
Nucleic acids research 2024 May 8;52(8):4215-4233
Intracellular TMEM16A is necessary for myogenesis of skeletal muscle.
Yuan W, Cui CC, Li J, Xu YH, Fan CE, Chen YC, Fan HW, Hu BX, Shi MY, Sun ZY, Wang P, Ma TX, Zhang Z, Zhu MS, Chen HQ
iScience 2022 Nov 18;25(11):105446
iScience 2022 Nov 18;25(11):105446
Switching in the expression pattern of actin isoforms marks the onset of contractility and distinct mechanodynamic behavior during cardiomyocyte differentiation.
Pires RH, Dau TH, Manu E, Shree N, Otto O
Physiological reports 2022 Feb;10(3):e15171
Physiological reports 2022 Feb;10(3):e15171
Nuclear miR-665 aggravates heart failure via suppressing phosphatase and tensin homolog transcription.
Fan J, Zhang X, Nie X, Li H, Yuan S, Dai B, Zhan J, Wen Z, Jiang J, Chen C, Wang D
Science China. Life sciences 2020 May;63(5):724-736
Science China. Life sciences 2020 May;63(5):724-736
Mechanical Ring Interfaces between Adherens Junction and Contractile Actomyosin to Coordinate Entotic Cell-in-Cell Formation.
Wang M, Niu Z, Qin H, Ruan B, Zheng Y, Ning X, Gu S, Gao L, Chen Z, Wang X, Huang H, Ma L, Sun Q
Cell reports 2020 Aug 25;32(8):108071
Cell reports 2020 Aug 25;32(8):108071
Optimization and enrichment of induced cardiomyocytes derived from mouse fibroblasts by reprogramming with cardiac transcription factors.
Tian J, Wang R, Hou Q, Li M, Chen L, Deng X, Zhu Z, Zhao Y, He W, Fu X
Molecular medicine reports 2018 Mar;17(3):3912-3920
Molecular medicine reports 2018 Mar;17(3):3912-3920
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Alpha-Actinin using anti- Alpha-Actinin antibody (MA1104). Alpha-Actinin was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1.5μg/ml mouse anti- Alpha-Actinin Antibody (MA1104) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
- Additional image
