MA1104

antibody from Boster Biological Technology

Targeting: ACTN2

Western blot (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1104

Provider

Boster Biological Technology

Product name

Anti-alpha-Actinin Antibody (monoclonal)

Antibody type

Monoclonal

Antigen

Rabbit skeletal alpha-actinin.

Description

Ascites

Reactivity

Human, Mouse, Rat, Rabbit

Vial size

100μg/vial

Concentration

Lyophilized

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100μg/ml.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Alpha-Actinin using anti- Alpha-Actinin antibody (MA1104). Alpha-Actinin was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1.5μg/ml mouse anti- Alpha-Actinin Antibody (MA1104) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Additional image