PA1-18346

antibody from Invitrogen Antibodies

Targeting: TRPV2 VRL, VRL-1, VRL1

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-18346

Provider

Invitrogen Antibodies

Product name

TRPV2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Molecular and Functional Study of Transient Receptor Potential Vanilloid 1-4 at the Rat and Human Blood-Brain Barrier Reveals Interspecies Differences.

Luo H, Saubamea B, Chasseigneaux S, Cochois V, Smirnova M, Glacial F, Perrière N, Chaves C, Cisternino S, Declèves X
Frontiers in cell and developmental biology 2020;8:578514

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 3 Protein expression of TRPV2 and TRPV4 in hCMEC/D3. (A) Expression of TRPV2 and TRPV4 determined by Western blot of total crude proteins obtained from two different protein extractions of hCMEC/D3. beta-actin served as a loading control housekeeping protein. (B) Confocal immunofluorescence localization of TRPV2 and TRPV4 in hCMEC/D3. TRPV2 or TRPV4 (green) and nuclei (blue) were stained in hCMEC/D3 as described in section ""Materials and Methods"" (second D, E, F and third G, H, I row panels). Claudin-5 (green) and nuclei (blue) were stained in hCMEC/D3 (fourth row panel J, K, M). VE-Cadherin (green) and nuclei (blue) were stained in hCMEC/D3 (fifth row panel N, O, P). Control cells (NEG) were stained omitting the primary antibodies (first row panel A, B, C).