Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27555 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPV2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.03 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VRL1 using 30 µg of A) A431 and B) Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a VRL1 polyclonal antibody (Product # PA5-27555) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VRL1 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a VRL1 polyclonal antibody (Product # PA5-27555) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TRPV2 Polyclonal Antibody (Product # PA5-27555). Unboiled K562 whole cell and membrane extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TRPV2 Polyclonal Antibody (Product # PA5-27555) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung, using VRL1 (Product # PA5-27555) antibody (10 µg/mL). Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.