PA5-52071

antibody from Invitrogen Antibodies

Targeting: NCOA6 AIB3, ASC2, KIAA0181, NRC, PRIP, RAP250, TRBP

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52071

Provider

Invitrogen Antibodies

Product name

NCOA6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: NQNVQHAGGQ GAGPPQNQMQ VSHGPPNMMQ PSLMGIHGNM NNQQAGTSGV PQVNLSNMQG QPQQGPPSQL MGMHQQIVPS QGQMVQQQGT LNPQNPMILS RAQLMPQGQM MVNPPSQNLG PSPQRMTPPK Q Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 92%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Nuclear receptor coactivator 6 promotes HTR-8/SVneo cell invasion and migration by activating NF-κB-mediated MMP9 transcription.

Wu L, Zhao KQ, Wang W, Cui LN, Hu LL, Jiang XX, Shuai J, Sun YP
Cell proliferation 2020 Sep;53(9):e12876

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NCOA6 in human cell line U-251 MG shows positivity in vesicles & nucleus but excluded from the nucleoli. Samples were probed using a NCOA6 Polyclonal Antibody (Product # PA5-52071).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NCOA6 in human testis and skeletal muscle tissues using NCOA6 Polyclonal Antibody (Product # PA5-52071). Corresponding NCOA6 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NCOA6 in human testis using NCOA6 Polyclonal Antibody (Product # PA5-52071) shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NCOA6 in human placenta using NCOA6 Polyclonal Antibody (Product # PA5-52071) shows moderate to strong nuclear positivity in trophoblastic cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NCOA6 in human fallopian tube using NCOA6 Polyclonal Antibody (Product # PA5-52071) shows moderate to strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NCOA6 in human skeletal muscle using NCOA6 Polyclonal Antibody (Product # PA5-52071) shows weak nuclear positivity in myocytes.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 NCOA6 knockdown significantly impaired the invasion and migration of HTR-8/SVneo cells. A, Significantly attenuated invasion and migration occurred after the transfection of NCOA6 siRNAs into HTR-8/SVneo cells. All invaded/migrated cells outside the insert membrane were stained and photographed (left panel; scale bars: 1 mm). The fold change in cell invasion/migration capacity after treatment was estimated by counting the invaded/migrated cells with staining (right panel). B, In parallel with the transwell assays, CCK-8 assays were carried out to determine the change in the number of cells 0, 24, 48 and 72 hours after transfection. C, D, The mRNA and protein levels of NCOA6 were examined after siRNA transfection. A representative image from a Western blot is shown, and the molecular weight markers are indicated on the left in kDa. All results are presented as the mean +- SD based on at least three independent experiments. ** P < .01