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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [3]
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Validation data
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- Product number
- LS-C2788 - Provider product page
- Provider
- LSBio
- Product name
- SLC2A10 / GLUT10 Antibody (aa367-385) LS-C2788
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NCI-H1299 non-small cell lung tumor cells were transfected with the eukaryotic expression vectors pcDNA3.1/V5 (lanes 1,2), pCMV-GLUT10 (lanes 3,4), and pCMV-GLUT1 (lanes 5,6). GLUT10 was detected using GLUT10 (Glucose Transporter 10) Antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NCI-H1299 non-small cell lung tumor cells were transfected with the eukaryotic expression vectors pcDNA3.1/V5 (lanes 1,2), pCMV-GLUT10 (lanes 3,4), and pCMV-GLUT1 (lanes 5,6). GLUT10 was detected using GLUT10 (Glucose Transporter 10) Antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Cochlea from embryonic mice and mice up to 1 year old were removed and the pure membrane fraction loaded on 4-20% SDS gel, Following electrophoresis, transfer and blocking, the blot was incubated overnight at 4 degrees C with the GLUT10 primary antibody at a 1:500 dilution followed by incubation with secondary antibody at a 1:3000 dilution for 1 h at room temperature. Protein bands were visualized on x-ray film with SuperSignal west Femto chemiluminescent substrate. Lanes contain 10 ug of protein.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NCI-H1299 non-small cell lung tumor cells were transfected with the eukaryotic expression vectors pcDNA3.1/V5 (lanes 1,2), pCMV-GLUT10 (lanes 3,4), and pCMV-GLUT1 (lanes 5,6). GLUT10 was detected.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- NCI-H1299 non-small cell lung tumor cells were transfected with the eukaryotic expression vectors pcDNA3.1/V5 (lanes 1,2), pCMV-GLUT10 (lanes 3,4), and pCMV-GLUT1 (lanes 5,6). GLUT10 was detected.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- NCI-H1299 non-small cell lung tumor cells were transfected with the eukaryotic expression vectors pcDNA3.1/V5 (lanes 1,2), pCMV-GLUT10 (lanes 3,4), and pCMV-GLUT1 (lanes 5,6). GLUT10 was detected.
