Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-46816 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-46816, RRID:AB_10009879
- Product name
- Rabbit Polyclonal EYA2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: EYA2 Antibody [NBP1-46816] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-EYA2 antibody NBP1-46816 used for IP at 3 ug/mg lysate. EYA2 was also immunoprecipitated by rabbit anti-EYA2 antibody NBP1-46817, which recognizes a downstream epitope. For blotting immunoprecipitated EYA2, NBP1-46817 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EYA2 Antibody [NBP1-46816] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EYA2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB