NBP1-46817

antibody from Novus Biologicals

Targeting: EYA2 EAB1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-46817

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-46817, RRID:AB_10009880

Product name

Rabbit Polyclonal EYA2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: EYA2 Antibody [NBP1-46817] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-EYA2 antibody NBP1-46817 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. EYA2 was also immunoprecipitated by rabbit anti-EYA2 antibody NBP1-46816, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: EYA2 Antibody [NBP1-46817] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: EYA2 Antibody [NBP1-46817] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EYA2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB