PA5-55401

antibody from Invitrogen Antibodies

Targeting: POLD2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-55401

Provider

Invitrogen Antibodies

Product name

POLD2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PRSKYIHPDD ELVLEDELQR IKLKGTIDVS KLVTGTVLAV FGSVRDDGKF LVEDYCFADL APQKPAPPLD TDRFVLLVSG LGLGGGGGES LLGTQLLVDV VTGQLGDEGE QC Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 96%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.08 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

H3.3K27M-induced chromatin changes drive ectopic replication through misregulation of the JNK pathway in C. elegans.

Delaney K, Strobino M, Wenda JM, Pankowski A, Steiner FA
Nature communications 2019 Jun 7;10(1):2529

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of POLD2 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a POLD2 Polyclonal Antibody (Product # PA5-55401).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 H3.3K27M mutation drives C. elegans germ cells towards a replicative fate. a Cartoon of H3.3K27M mutation, and boxplot showing fertility levels of wild-type and H3.3K27M mutant (mut) worms at 25 degC. N = 5 for wt, N = 4 for mut. b Cartoon images of worms and germ lines highlighting different stages of germcell development in wild-type and mut worms. DAPI-stained adult gonads of wild-type (left) and mut (right) worms. Distal tip (asterisk), normal (arrows), and endomitotic (arrowheads) oocytes are highlighted. A representative normal (wild type) and endomitotic (mut) oocyte is enlarged. Scale bars represent 75 um on whole gonad pictures and 20 um on enlarged oocyte pictures. c Boxplot showing the number of normal and endomitotic oocytes per gonad in wild-type and mut worms. N = 7 for wt, N = 9 for mut. d Boxplot showing relative DNA content in endomitotic oocytes compared with wild-type oocytes (diakinesis I, 4 n) based on the DAPI quantification. N = 10. e H3.3K27M mutant late pachytene and diakinesis germ cells are positive for markers of replication (DNA polymerase delta subunit 2 (POLD2) and BrdU incorporation), mitosis (H3 phosphoS10) and DNA double stranded breaks (RAD-51). POLD2 staining in pachytene nuclei as well as POLD2, BrdU, H3 phosphoS10, and RAD-51 staining in normal oocytes of wild-type and endomitotic oocytes of mut worms are shown. Scale bars equal 15 um for late pachytene nuclei images and 10 um for wild-type and endomitotic ooc