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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [5]
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- Product number
- LS-C344069 - Provider product page
- Provider
- LSBio
- Product name
- HNF4A / HNF4 Antibody (aa164-474) LS-C344069
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- HNF-4-alpha antibody Western blot. All lanes: Anti HNF-4-alpha at 0.5 ug/ml. WB: Recombinant Human HNF-4-alpha Protein 0.5ng. Predicted band size: 38 kD. Observed band size: 38 kD.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- HNF-4-alpha antibody Western blot. All lanes: Anti HNF-4-alpha at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Lane 3: RH35 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Lane 5: HEPG2 Whole Cell Lysate at 40 ug. Predicted band size: 53 kD. Observed band size: 53 kD.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- HNF-4-alpha antibody IHC-paraffin: Rat Kidney Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- HNF-4-alpha antibody IHC-paraffin: Mouse Kidney Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- HNF-4-alpha antibody IHC-paraffin: Human Intestinal Cancer Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC analysis of HNF-4-alpha using anti-HNF-4-alpha antibody. HNF-4-alpha was detected in frozen section of Mouse Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-HNF-4-alpha Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC analysis of HNF-4-alpha using anti-HNF-4-alpha antibody. HNF-4-alpha was detected in frozen section of Rat Kindey Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-HNF-4-alpha Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
