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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-36857 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-HNF4A (Ser313) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 52 kDa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-HNF4 alpha pSer313 using Phospho-HNF4 alpha pSer313 polyclonal antibody (Product # PA5-36857) at a dilution of 1:500. Lane 1: Hela cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 2: Raw264.7 cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 3: PC12 cell lysate treated with EGF (0.1 ng/mL, 30 min).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1%SDS) (30 µg lysate) of Hep G2 (Lane 1) and Hep G2 treated with EGF (200 ng/mL for 30 minutes) (Lane 2). The blot was probed with Anti-Phospho-HNF4A (Ser313) Polyclonal Antibody (Product # PA5-36857, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 56 kDa band corresponding to Phospho-HNF4A (Ser313) was induced in Hep G2 cell line treated with EGF.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-HNF4 alpha pSer313 in paraffin-embedded human liver carcinoma using Phospho-HNF4 alpha pSer313 polyclonal antibody (Product # PA5-36857).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-HNF4A (Ser313) in paraffin-embedded human liver cancer tissue. Samples were incubated with Phospho-HNF4A (Ser313) polyclonal antibody (Product # PA5-36857).
