H00003172-M04-0.05mg

antibody from Novus Biologicals

Targeting: HNF4A HNF4, MODY, MODY1, NR2A1, TCF14

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00003172-M04-0.05mg

Provider

Novus Biologicals

Product name

Mouse Monoclonal HNF-4 alpha/NR2A1 Antibody

Antibody type

Monoclonal

Description

IgG purified. HNF4A (4E2)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.05 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi or non-transfected control. Blot probed with H00003172-M04. GAPDH (36.1 kDa) used as loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody (M04), clone 4E2. Lane 1: HNF4A transfected lysatE (51.6 KDa). Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Analysis of HNF4A expression in Jurkat (Cat # L017V1).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Analysis of HNF4A expression in HepG2 (Cat # L019V1).

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Detection limit for recombinant GST tagged HNF4A is approximately 1ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: HNF-4 alpha/NR2A1 Antibody (4E2) [H00003172-M04] - Analysis of monoclonal antibody to HNF4A on HeLa cell. Antibody concentration 10 ug/ml