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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- R31628 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Hnf4a Antibody
- Antibody type
- Polyclonal
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HNF4A antibody and Lane 1: rat liver; 2: (r) kidney; 3: (r) RH35; 4: human HeLa; 5: (h) HEPG2 lysate. Predicted molecular weight ~50kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HNF4A antibody and recombinant human protein (0.5ng)
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of FFPE human HepG2 cells with HNF4A antibody (2ug/ml) and DAPI counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: HNF4A antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: HNF4A antibody testing of mouse kidney tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P testing of rat kidney tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-F: HNF4A antibody testing of mouse liver tissue.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-F: HNF4A antibody testing of rat kidney tissue.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HepG2 cells with HNF4A antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HNF4A antibody.
