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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [5]
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- Product number
- R31628 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HNF4A Antibody
- Antibody type
- Polyclonal
- Antigen
- Human partial recombinant protein (AA 164-474) was used as the immunogen for this HNF4A antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the HNF4A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HNF4A antibody and Lane 1: rat liver; 2: (r) kidney; 3: (r) RH35; 4: human HeLa; 5: (h) HEPG2 lysate. Predicted molecular weight ~50kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HNF4A antibody and recombinant human protein (0.5ng)
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: HNF4A antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: HNF4A antibody testing of mouse kidney tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P testing of rat kidney tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-F: HNF4A antibody testing of mouse liver tissue.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-F: HNF4A antibody testing of rat kidney tissue.
