Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00003172-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003172-D01, RRID:AB_10717224
- Product name
- HNF4A MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human HNF4A protein.
- Antigen sequence
MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLT
MGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGK
HYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVD
KDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTR
RSSYEDSSLPSINALLQAEVLSRQITSPVSGINGD
IRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCE
LPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLL
GNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQ
IDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQV
QVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQ
MIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHA
HHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMSTP
ETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQ
PTITKQEVI- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HNF4A MaxPab rabbit polyclonal antibody. Western Blot analysis of HNF4A expression in HeLa.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of HNF4A expression in transfected 293T cell line (H00003172-T01) by HNF4A MaxPab polyclonal antibody.Lane 1: HNF4A transfected lysate(51.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of HNF4A transfected lysate using anti-HNF4A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HNF4A purified MaxPab mouse polyclonal antibody (B01P) (H00003172-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol