M00389

antibody from Boster Biological Technology

Targeting: HNF4A HNF4, MODY, MODY1, NR2A1, TCF14

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

M00389

Provider

Boster Biological Technology

Product name

Anti-HNF-4-alpha Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for NR2A1/HNF4A detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human NR2A1/HNF4A information: Molecular Weight: 52785 MW; Subcellular Localization: Nucleus.

Reactivity

Human

Host

Rabbit

Antibody clone number

GBA-8

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of HNF-4-alpha expression in A549 cell lysate (M00389). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF4A monoclonal antibody (Catalog # M00389) overnight at 4

Additional image