Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-1783 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1783, RRID:AB_10001816
- Product name
- Goat Polyclonal HNF-4 alpha/NR2A1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1
- Reactivity
- Human, Mouse, Simian
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Estrogen receptor α represses transcription of HBV genes via interaction with hepatocyte nuclear factor 4α.
Novel P2 promoter-derived HNF4alpha isoforms with different N-terminus generated by alternate exon insertion.
Novel P2 promoter-derived HNF4alpha isoforms with different N-terminus generated by alternate exon insertion.
Expression of HNF4alpha variants in pancreatic islets and Ins-1 beta cells.
Polymorphisms in both promoters of hepatocyte nuclear factor 4-alpha are associated with type 2 diabetes in the Amish.
Polymorphisms in both promoters of hepatocyte nuclear factor 4-alpha are associated with type 2 diabetes in the Amish.
Wang SH, Yeh SH, Lin WH, Yeh KH, Yuan Q, Xia NS, Chen DS, Chen PJ
Gastroenterology 2012 Apr;142(4):989-998.e4
Gastroenterology 2012 Apr;142(4):989-998.e4
Novel P2 promoter-derived HNF4alpha isoforms with different N-terminus generated by alternate exon insertion.
Huang J, Levitsky LL, Rhoads DB
Experimental cell research 2009 Apr 15;315(7):1200-11
Experimental cell research 2009 Apr 15;315(7):1200-11
Novel P2 promoter-derived HNF4alpha isoforms with different N-terminus generated by alternate exon insertion.
Huang J, Levitsky LL, Rhoads DB
Experimental cell research 2009 Apr 15;315(7):1200-11
Experimental cell research 2009 Apr 15;315(7):1200-11
Expression of HNF4alpha variants in pancreatic islets and Ins-1 beta cells.
Huang J, Karakucuk V, Levitsky LL, Rhoads DB
Diabetes/metabolism research and reviews 2008 Oct;24(7):533-43
Diabetes/metabolism research and reviews 2008 Oct;24(7):533-43
Polymorphisms in both promoters of hepatocyte nuclear factor 4-alpha are associated with type 2 diabetes in the Amish.
Damcott CM, Hoppman N, Ott SH, Reinhart LJ, Wang J, Pollin TI, O'Connell JR, Mitchell BD, Shuldiner AR
Diabetes 2004 Dec;53(12):3337-41
Diabetes 2004 Dec;53(12):3337-41
Polymorphisms in both promoters of hepatocyte nuclear factor 4-alpha are associated with type 2 diabetes in the Amish.
Damcott CM, Hoppman N, Ott SH, Reinhart LJ, Wang J, Pollin TI, O'Connell JR, Mitchell BD, Shuldiner AR
Diabetes 2004 Dec;53(12):3337-41
Diabetes 2004 Dec;53(12):3337-41
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HNF-4 alpha/NR2A1 Antibody [NB100-1783] - (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HNF-4 alpha/NR2A1 Antibody [NB100-1783] - (0.5ug/ml) staining of Mouse Small Intestine lysate (35ug protein in RIPA buffer). . Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NB100-1783] - Staining of Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.