AF4605

antibody from R&D Systems

Targeting: HNF4A HNF4, MODY, MODY1, NR2A1, TCF14

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4605

Provider

R&D Systems

Product name

Human HNF-4 alpha/NR2A1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human HNF-4 alpha/NR2A1 in Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P41235


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human HNF-4 alpha/NR2A1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human HNF-4 alpha/NR2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band for HNF-4 alpha/NR2A1 was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human HNF-4 alpha/NR2A1 by Simple Western^TM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HNF-4 alpha/NR2A1 at approximately 60 kDa (as indicated) using 50 µg/mL of Goat Anti-Human HNF-4 alpha/NR2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4605) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.