LS-C672909

antibody from LSBio

Targeting: POLDIP3 KIAA1649, PDIP3, PDIP46, SKAR

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C672909

Provider

LSBio

Product name

POLDIP3 / p46 Antibody LS-C672909

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate All lanes: POLDIP3 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 43 kDa Observed band size: 43 kDa

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate. All lanes: POLDIP3 antibody at 3.2 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 43 KDa. Observed band size: 47 KDa

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate. All lanes: POLDIP3 antibody at 3.2 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 43 KDa. Observed band size: 47 KDa

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate All lanes: POLDIP3 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 43 kDa Observed band size: 43 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating POLDIP3 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of POLDIP3 Antibody in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: POLDIP3 Antibody (8µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating POLDIP3 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of POLDIP3 Antibody in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: POLDIP3 Antibody (8µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating POLDIP3 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of POLDIP3 Antibody in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: POLDIP3 Antibody (8µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (10µg)