PA5-21998

antibody from Invitrogen Antibodies

Targeting: PRKAG2 AAKG, AAKG2, CMH6, H91620p, WPWS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21998

Provider

Invitrogen Antibodies

Product name

AMPK gamma-2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: HepG2.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AMPK gamma-2 using 50 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an AMPK gamma-2 polyclonal antibody (Product # PA5-21998) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AMPK gamma-2 using 30 µg of HeLa S3 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an AMPK gamma-2 polyclonal antibody (Product # PA5-21998) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of AMPK gamma-2 was performed by separating 30 µg of whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a AMPK gamma-2 Polyclonal Antibody (Product # PA5-21998) at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of AMPK gamma-2 in paraformaldehyde-fixed HeLa cells using an AMPK gamma-2 polyclonal antibody (Product # PA5-21998) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AMPK gamma-2 antibody (Product # PA5-21998) at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AMPK gamma-2 Polyclonal Antibody detects PRKAG2 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer tissue. AMPK gamma-2 Polyclonal Antibody (Product # PA5-21998) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.