PA5-22331

antibody from Invitrogen Antibodies

Targeting: PRKAG2 AAKG, AAKG2, CMH6, H91620p, WPWS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22331

Provider

Invitrogen Antibodies

Product name

AMPK gamma-2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, Mouse brain.

Concentration

0.96 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AMPK gamma-2 using 30 µg of HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an AMPK gamma-2 polyclonal antibody (Product # PA5-22331) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of AMPK gamma-2 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a AMPK gamma-2 Polyclonal Antibody (Product # PA5-22331) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using AMPK gamma-2 Polyclonal Antibody (Product # PA5-22331). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. AMPK gamma-2 Polyclonal Antibody (Product # PA5-22331) diluted at 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AMPK gamma-2 Polyclonal Antibody detects PRKAG2 protein at nucleus and cytosol human on hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. AMPK gamma-2 Polyclonal Antibody (Product # PA5-22331) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.