PA5-88333

antibody from Invitrogen Antibodies

Targeting: SEL1L Hrd3, IBD2, SEL1L1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88333

Provider

Invitrogen Antibodies

Product name

SEL1L Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SSDEEGSQDE SLDSKTTLTS DESVKDHTTA GRVVAGQIFL DSEESELESS IQEEEDSLKS QEGESVTEDI SFLESPNPEN KDYEEPKKVR KPALTAIEGT AHGEPCHFPF LFLDKEYDEC TSDGREDGRL WCATTYDYKA DEKWGFCETE EEAAKRRQMQ EAEMMYQTG; Positive Samples: BxPC-3, HepG2, 293T, Mouse kidney, Rat liver; Cellular Location: Endoplasmic reticulum membrane, Single-pass type I membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.78 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SEL1L in various lysates. Samples were incubated with SEL1L Polyclonal antibody (Product # PA5-88333) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of SEL1L in C6 cells. Samples were incubated with SEL1L Polyclonal antibody (Product # PA5-88333) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SEL1L was performed in L929 cells using SEL1L Polyclonal Antibody (Product # PA5-88333).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of SEL1L in L929 cells. Samples were incubated with SEL1L Polyclonal antibody (Product # PA5-88333) using a dilution of 1:100. Blue: DAPI for nuclear staining.