PA5-24179

antibody from Invitrogen Antibodies

Targeting: SEL1L Hrd3, IBD2, SEL1L1

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-24179

Provider

Invitrogen Antibodies

Product name

SEL1L Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The Application of the Tissue Microarray (TMA) Technology to Analyze Cerebral Organoids.

Biunno I, Paiola E, De Blasio P
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2021 Jul;69(7):451-460

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SEL1L plays a major role in human malignant gliomas.

Mellai M, Annovazzi L, Boldorini R, Bertero L, Cassoni P, De Blasio P, Biunno I, Schiffer D
The journal of pathology. Clinical research 2020 Jan;6(1):17-29

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Sel1l knockdown negatively influences zebrafish embryos endothelium.

Barbieri A, Carra S, De Blasio P, Cotelli F, Biunno I
Journal of cellular physiology 2018 Jul;233(7):5396-5404

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of MCF-7 cells using a SEL1L polyclonal antibody (Product # PA5-24179) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Immunohistochemistry in malignant gliomas with monoclonal and polyclonal anti-SEL1L antibodies. (A) Anaplastic astrocytoma, SEL1LA+ neurons, microglia/macrophages and mostly tumour cells; DAB, original magnification (OM) x 200. (B) Anaplastic astrocytoma, SEL1L-p70/80+ neurons, microglia/macrophages and mostly tumour cells; DAB, OM x 200. (C) Anaplastic oligodendroglioma, SEL1LA+ tumour cells in the cytoplasm and nucleus; DAB, OM x 200. (D) Anaplastic oligodendroglioma, SEL1L-p70/80+ tumour cells in the nucleus; DAB, OM x 200. (E) Anaplastic oligodendroglioma, SEL1LA+ tumour cells; DAB, OM x 200. (F) Anaplastic oligodendroglioma, Ki-67/MIB-1+ cells; DAB, OM x 200. (G) GB, H&E; DAB, OM x 400. (H) GB, SEL1LA+ tumour cells in the cytoplasm and nuclei; DAB, OM x 400; (I) GB, SEL1L-p70/80+ tumour cells in the nucleus; DAB, OM x 400. (J) GB, SEL1L-p70/80+ tumour and myeloid cells around a perinecrotic pseudopalisade; DAB, OM x 200. (K) GB, Nestin+ tumour cells; DAB, OM x 400. (L) GB, Sox2+ tumour cells; DAB, OM x 400. (M) GB, Ki-67/MIB-1+ cells; DAB, OM x 400. DAB, 3,3'-diaminobenzidine.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Immunohistochemistry using monoclonal and polyclonal anti-SEL1L antibodies with regard to tumour neo-vasculature and GAMs. (A) GB with proliferated tumour blood vessels, SEL1LA+ ECs and vascular pericytes; DAB, original magnification (OM) x 200. (B) GB with proliferated tumour blood vessels, SEL1L-p70/80+ ECs and pericytes; DAB, OM x 200. (C) GB with proliferated tumour blood vessels, SEL1LA+ and alpha-SM-Actin+ vascular pericytes; double immunohistochemistry with DAB and Alkaline Phosphatase Red, respectively, OM 200x. (D) GB with proliferated tumour blood vessels, NG2/CSPG4+ ECs and pericytes; DAB, OM x 200. (E) Diffuse astrocytoma, Iba-1+ GAMs; DAB, OM x 200. (F) Pilocytic astrocytoma, Iba-1+ GAMs; DAB, OM x 200. (G) Diffuse oliodendroglioma, Iba-1+ GAMs; DAB, OM x 200. (H) Anaplastic astrocytoma, Iba-1+ GAMs; DAB, OM x 200. (I) Anaplastic oligodendroglioma, Iba-1+ GAMs; DAB, OM x 200. (J) GB, Iba-1+ scattered macrophages in tumour parenchyma; DAB, OM x 200. (K) GB, SEL1LA+ scattered macrophages in tumour parenchyma; DAB, OM x 200. (L) GB, SEL1L-p70/80+ peri- and intravascular macrophages; DAB, OM x 200. DAB, 3,3'-diaminobenzidine.