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LearnSTJ99185
antibody from St John's Laboratory
Targeting: PRKAB1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- STJ99185 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-PRKAB1 antibody [1A7-E11-E9] (STJ99185)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-5 Amp-Activated Protein Kinase Subunit Beta-1 is suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry research applications.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of AMPK beta 1 in 3T3, Hela, PC-12, COS7 and MDA-MB-468 cell lysates using AMPK beta 1 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using AMPK beta 1 mouse mAb (dilution 1:100).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Hela cell lysates using AMPK beta 1 mouse mAb.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
