Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 649801 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#649801, RRID:AB_10681722
- Product name
- Purified anti-AMPKĀ
- Antibody type
- Polyclonal
- Antigen
- AMPK catalytic subunit (α subunit)
- Description
- AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. AMPK is a heterodimeric protein serine/threonine kinase that is composed of alpha- (catalytic) and beta/gamma- (regulatory) subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. This catalytic subunit contains a classical serine/threonine protein kinase domain close to the N-terminal. Free catalytic α subunits are usually inactive due to the presence of an autoinhibitory domain that is located in the center of this subunit. When the α subunit forms a functional complex with the β and γ subunits, this autoinhibitory function no longer blocks the catalytic function. In response to cellular metabolic stresses, AMPK is activated in an AMP-dependent manner by targeted phosphorylation of Thr172 in the α subunit by the upstream kinase like LKB1, calmodulin-dependent protein kinase kinases and transforming growth factor-β-activated kinase-1. Activated AMPK phosphorylates many metabolic enzymes to stimulate catabolic pathways, such as ketogenesis, and inhibit anabolic pathways, such as protein synthesis. Recent studies also suggest the important role of AMPK in regulating chemotaxis and cytokine secretion in immune cells.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 50 µl
- Storage
- Upon receipt, store undiluted at -20°C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- 293T cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified polyclonal anti-AMPK antibody. Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated