MA1-25102

antibody from Invitrogen Antibodies

Targeting: NR3C1 GR, GRL

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-25102

Provider

Invitrogen Antibodies

Product name

Glucocorticoid Receptor Monoclonal Antibody (8E9)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is not suitable for IHC applications. Predicted molecular weight is 93.24 kDa.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

8E9

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Glucocorticoid Receptor was performed using MDA-MB-231 cells (serum-starved) and MDA-MB-231 cells serum-starved for 24 hours, followed by 1 µM Dexamethasone treatment for 2 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Glucocorticoid Receptor Monoclonal Antibody (8E9) (Product # MA1-25102) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green) in MDA-MB-231 treated cells. Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization of NR3C1 protein in MDA-MB-231 treated cells. Panel e represents the merged image of MDA-MB-231 untreated cells, that shows cytoplasmic localization of NR3C1 protein. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.