Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA1-25102 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Glucocorticoid Receptor Monoclonal Antibody (8E9)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is not suitable for IHC applications. Predicted molecular weight is 93.24 kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8E9
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Glucocorticoid Receptor was performed using MDA-MB-231 cells (serum-starved) and MDA-MB-231 cells serum-starved for 24 hours, followed by 1 µM Dexamethasone treatment for 2 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Glucocorticoid Receptor Monoclonal Antibody (8E9) (Product # MA1-25102) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green) in MDA-MB-231 treated cells. Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization of NR3C1 protein in MDA-MB-231 treated cells. Panel e represents the merged image of MDA-MB-231 untreated cells, that shows cytoplasmic localization of NR3C1 protein. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.