MA1-81793

antibody from Invitrogen Antibodies

Targeting: NR3C1 GR, GRL

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-81793

Provider

Invitrogen Antibodies

Product name

Glucocorticoid Receptor Monoclonal Antibody (5E4), FITC

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Conjugate

Green dye

Antibody clone number

5E4

Concentration

0.1 mg/mL

References

Submitted references

Glucocorticoid Receptor Expression in Peripheral WBCs of Critically Ill Children.

Shibata AR, Troster EJ, Wong HR
Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies 2015 Jun;16(5):e132-40

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Glucocorticoid Receptor was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Glucocorticoid Receptor, FITC Mouse Monoclonal Antibody (5E4) (Product # MA1-81793) at 5 µg/mL in 0.1% BSA and incubated at 4 degree Celsius overnight (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents isotype control to assess background. The images were captured at 60X magnification.

Conjugate

Green dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. Glucocorticoid receptor (GCR) expression in CD4 lymphocytes, CD8 lymphocytes, monocytes (CD14), and neutrophils (CD66b) in patients with cardiovascular (CV) failure and without CV failure. GCR expression was lower in patients with CV failure when compared with patients without CV failure, p < 0.05 by rank-sum test. MFI = mean fluorescence intensity.

Conjugate

Green dye