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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [5]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX101120 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101120, RRID:AB_1951023
- Product name
- Glucocorticoid Receptor antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Antiviral and anti-inflammatory activity of budesonide against human rhinovirus infection mediated via autophagy activation.
Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer.
Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-α protein levels and activity.
Glucocorticoid receptor β regulates injury-mediated astrocyte activation and contributes to glioma pathogenesis via modulation of β-catenin/TCF transcriptional activity.
Coactivators enable glucocorticoid receptor recruitment to fine-tune estrogen receptor transcriptional responses.
Kim SR, Song JH, Ahn JH, Lee GS, Ahn H, Yoon SI, Kang SG, Kim PH, Jeon SM, Choi EJ, Shin S, Cha Y, Cho S, Kim DE, Chang SY, Ko HJ
Antiviral research 2018 Mar;151:87-96
Antiviral research 2018 Mar;151:87-96
Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
Wu YL, Chang JC, Lin WY, Li CC, Hsieh M, Chen HW, Wang TS, Wu WT, Liu CS, Liu KL
Free radical biology & medicine 2018 Feb 1;115:309-317
Free radical biology & medicine 2018 Feb 1;115:309-317
A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer.
Choi EJ, Jung BJ, Lee SH, Yoo HS, Shin EA, Ko HJ, Chang S, Kim SY, Jeon SM
Oncogene 2017 Sep 14;36(37):5285-5295
Oncogene 2017 Sep 14;36(37):5285-5295
Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-α protein levels and activity.
Bolt MJ, Stossi F, Callison AM, Mancini MG, Dandekar R, Mancini MA
Oncogene 2015 Jan 8;34(2):154-64
Oncogene 2015 Jan 8;34(2):154-64
Glucocorticoid receptor β regulates injury-mediated astrocyte activation and contributes to glioma pathogenesis via modulation of β-catenin/TCF transcriptional activity.
Yin Y, Zhang X, Li Z, Deng L, Jiao G, Zhang B, Xie P, Mu H, Qiao W, Zou J
Neurobiology of disease 2013 Nov;59:165-76
Neurobiology of disease 2013 Nov;59:165-76
Coactivators enable glucocorticoid receptor recruitment to fine-tune estrogen receptor transcriptional responses.
Bolt MJ, Stossi F, Newberg JY, Orjalo A, Johansson HE, Mancini MA
Nucleic acids research 2013 Apr;41(7):4036-48
Nucleic acids research 2013 Apr;41(7):4036-48
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 7.5% SDS PAGE GTX101120 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glucocorticoid receptor antibody detects Glucocorticoid receptor protein by western blot analysis. Mouse tissue extracts (50 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody (GTX101120) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glucocorticoid receptor antibody detects Glucocorticoid receptor protein by western blot analysis. Rat tissue extracts (50 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody (GTX101120) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Glucocorticoid receptor knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody (GTX101120) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Glucocorticoid receptor knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody (GTX101120) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glucocorticoid receptor antibody immunoprecipitates Glucocorticoid receptor protein in IP experiments. IP Sample: 1000 ?g HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2.5 £gg of preimmune rabbit IgG C. Immunoprecipitation of Glucocorticoid receptor protein by 2.5 £gg of Glucocorticoid receptor antibody (GTX101120) 7.5% SDS-PAGE The immunoprecipitated Glucocorticoid receptor protein was detected by SCMH1 antibody (GTX101120) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Glucocorticoid receptor(GTX101120) antibody at 1:500 dilution.
