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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324810 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal NR3C1 Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal NR3C1 Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- NR3C1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- NR3C1 Antibody (Center) (Cat.#TA324810) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the NR3C1 antibody detected NR3C1 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with NR3C1 Antibody (Center)(Cat#TA324810).Hela cells were incubated with NR3C1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . NR3C1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- NR3C1 Antibody (Center) (Cat. #TA324810)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR3C1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
