SP5211P

antibody from Acris Antibodies GmbH

Targeting: NR3C1 GR, GRL

Immunoprecipitation

Immunoelectron microscopy

Gel shift

Antibody data

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Validation data

Product number: SP5211P - Provider product page
Provider: Acris Antibodies GmbH
Proper citation: Acris Antibodies GmbH Cat#SP5211P, RRID:AB_1001830
Product name: anti Glucocorticoid receptor
Antibody type: Polyclonal
Antigen: Synthetic Peptide corresponding to amino acid residues 150-175 from Human GR.
Reactivity: Human, Rat
Host: Rabbit
Vial size: 0.2 ml

NR3C1 protein structure - SP5211P shown in red.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunofluorescent analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody Cat.-NoÂ SP5211PÂ shows staining in Â HeLa Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizingÂ Glucocorticoid Receptor Cat.-NoÂ SP5211P at a dilution of 1/100 over night at 4Â°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images wee taken at 60x magnification.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunofluorescent analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody Cat.-NoÂ SP5211PÂ shows staining in Â A2058 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizingÂ Glucocorticoid Receptor Cat.-NoÂ SP5211P at a dilution of 1/100 over night at 4Â°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images wee taken at 60x magnification.

Supportive validation

Submitted by: Acris Antibodies GmbH (provider)
Main image
Experimental details: Immunofluorescent analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody Cat.-No SP5211PÂ shows staining in 293Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizingÂ Glucocorticoid ReceptorÂ Cat.-NoÂ SP5211P at a dilution of 1/100 over night at 4Â°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images wee taken at 60x magnification.