GTX22768
antibody from GeneTex
Targeting: NR3C1
GR, GRL
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX22768 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX22768, RRID:AB_370718
- Product name
- Glucocorticoid Receptor antibody [BuGR2]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Guinea Pig, Rabbit, Sheep, Simian, Xenopus, Yeast
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of glucocorticoid receptor in mouse liver extract.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Glucocorticoid Receptor using in HeLa cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Glucocorticoid Receptor antibody [BuGR2] at a dilution of 1:100 over night at 4 ¢XC, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Glucocorticoid Receptor in HeLa cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant, adding 90% methanol and incubated for 10 minutes at room temperature. Follwing penetration, cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with Glucocorticoid Receptor antibody [BuGR2] at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a proper secondary antibody and re-suspended in PBS for FACS analysis.