NB300-731

antibody from Novus Biologicals

Targeting: NR3C1 GR, GRL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Gel shift (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB300-731

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-731, RRID:AB_2298869

Product name

Mouse Monoclonal GR/NR3C1 Antibody

Antibody type

Monoclonal

Description

Protein A purified. Using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.

Reactivity

Human, Mouse, Rat, Guinea Pig, Rabbit, Sheep, Yeast

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

In utero Exposure to Maternal Chronic Inflammation Transfers a Pro-Inflammatory Profile to Generation F2 via Sex-Specific Mechanisms.

Adams RCM, Smith C
Frontiers in immunology 2020;11:48

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Chronic Gestational Inflammation: Transfer of Maternal Adaptation over Two Generations of Progeny.

Adams RCM, Smith C
Mediators of inflammation 2019;2019:9160941

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Single-Cell Resolution and Quantitation of Targeted Glucocorticoid Delivery in the Thymus.

Taves MD, Mittelstadt PR, Presman DM, Hager GL, Ashwell JD
Cell reports 2019 Mar 26;26(13):3629-3642.e4

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Inhibitory Effects of Prunella vulgaris L. Extract on 11β-HSD1 in Human Skin Cells.

Roh KB, Park D, Jung E
Evidence-based complementary and alternative medicine : eCAM 2018;2018:1762478

---

Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels.

Rué L, Bañez-Coronel M, Creus-Muncunill J, Giralt A, Alcalá-Vida R, Mentxaka G, Kagerbauer B, Zomeño-Abellán MT, Aranda Z, Venturi V, Pérez-Navarro E, Estivill X, Martí E
The Journal of clinical investigation 2016 Nov 1;126(11):4319-4330

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Functionally distinct patterns of nucleosome remodeling at enhancers in glucocorticoid-treated acute lymphoblastic leukemia.

Wu JN, Pinello L, Yissachar E, Wischhusen JW, Yuan GC, Roberts CWM
Epigenetics & chromatin 2015;8:53

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Behavioural consequences of p-glycoprotein deficiency in mice, with special focus on stress-related mechanisms.

Schoenfelder Y, Hiemke C, Schmitt U
Journal of neuroendocrinology 2012 May;24(5):809-17

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Steroid hormones induce bcl-X gene expression through direct activation of distal promoter P4.

Viegas LR, Vicent GP, Barañao JL, Beato M, Pecci A
The Journal of biological chemistry 2004 Mar 12;279(11):9831-9

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Evidence for glucocorticoid receptor transport on microtubules by dynein.

Harrell JM, Murphy PJ, Morishima Y, Chen H, Mansfield JF, Galigniana MD, Pratt WB
The Journal of biological chemistry 2004 Dec 24;279(52):54647-54

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CBP recruitment and histone acetylation in differential gene induction by glucocorticoids and progestins.

Lambert JR, Nordeen SK
Molecular endocrinology (Baltimore, Md.) 2003 Jun;17(6):1085-94

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All of the factors required for assembly of the glucocorticoid receptor into a functional heterocomplex with heat shock protein 90 are preassociated in a self-sufficient protein folding structure, a "foldosome".

Hutchison KA, Dittmar KD, Pratt WB
The Journal of biological chemistry 1994 Nov 11;269(45):27894-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in NIH/3T3 cells compared to an isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in HeLa cells compared to an isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of GR in Jurkat cells compared to an isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - An intracellular stain was performed on Jurkat cells with GR/NR3C1 (BuGR2) antibody NB300-731PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerthrin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.