NB300-610

antibody from Novus Biologicals

Targeting: NR3C1 GR, GRL

Western blot

Chromatin Immunoprecipitation

Antibody data

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Validation data

NR3C1 protein structure - NB300-610 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody [NB300-610] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody shows staining in Hela Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody [NB300-610] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody [NB300-610] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody shows staining in NIH-3T3 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.