NBP1-81035

antibody from Novus Biologicals

Targeting: MPRIP M-RIP, p116Rip, RHOIP3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-81035

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-81035, RRID:AB_11035939

Product name

Rabbit Polyclonal MPRIP Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse, rat MPRIP antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

p116^Rip promotes myosin phosphatase activity in airway smooth muscle cells.

Komatsu S, Wang L, Seow CY, Ikebe M
Journal of cellular physiology 2020 Jan;235(1):114-127

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Dual role for DOCK7 in tangential migration of interneuron precursors in the postnatal forebrain.

Nakamuta S, Yang YT, Wang CL, Gallo NB, Yu JR, Tai Y, Van Aelst L
The Journal of cell biology 2017 Dec 4;216(12):4313-4330

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Genome-wide identification of miR-200 targets reveals a regulatory network controlling cell invasion.

Bracken CP, Li X, Wright JA, Lawrence DM, Pillman KA, Salmanidis M, Anderson MA, Dredge BK, Gregory PA, Tsykin A, Neilsen C, Thomson DW, Bert AG, Leerberg JM, Yap AS, Jensen KB, Khew-Goodall Y, Goodall GJ
The EMBO journal 2014 Sep 17;33(18):2040-56

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MPRIP Antibody [NBP1-81035] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MPRIP Antibody [NBP1-81035] - Analysis in human cell line PC-3.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MPRIP Antibody [NBP1-81035] - Staining of human liver shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MPRIP Antibody [NBP1-81035] - Staining of human smooth muscle shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MPRIP Antibody [NBP1-81035] - Staining in human smooth muscle and liver tissues using anti-MPRIP antibody. Corresponding MPRIP RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MPRIP Antibody [NBP1-81035] - Staining of human cerebral cortex.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MPRIP Antibody [NBP1-81035] - Staining of human testis.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MPRIP Antibody [NBP1-81035] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MPRIP Antibody [NBP1-81035] - Staining of human cerebral cortex, fallopian tube, liver and prostate using Anti-MPRIP antibody NBP1-81035 (A) shows similar protein distribution across tissues to independent antibody NBP1-81036 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MPRIP Antibody [NBP1-81035] - Staining of human Fallopian tube shows moderate positivity in apical membrane in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MPRIP Antibody [NBP1-81035] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MPRIP Antibody [NBP1-81035] - Staining of human prostate shows weak to moderate cytoplasmic positivity in smooth muscle cells.